Roma began their return to the Champions League after seven years with an opening fixture against Fenerbahçe in Istanbul on 10 September. Midfielder Bryan Cristante praised the team's performance despite the result not going their way, describing the display as "excellent" and highlighting Roma's control from the first minute. Cristante noted that Roma went ahead early but conceded an equaliser, leaving them with a draw. He emphasised the team's attacking intent, particularly in the second half, while acknowledging disappointment at conceding. "We managed the match well from the first minute," Cristante said. "In the second we attacked almost constantly. It's a shame about the goal we conceded, but we put in an excellent performance." Approximately 400 Roma supporters travelled to the Şükrü Saraçoğlu stadium in the Kadıköy neighbourhood on Istanbul's Asian shore, described by Roma News as one of European football's most hostile environments.
Manager Gian Piero Gasperini made his Roma debut in the competition, viewing the Champions League as central to his ambitions at the club. Gasperini confirmed Paulo Dybala was fit and central to Roma's hopes, stating: "He is already demonstrating what his consistency is, because his value is recognised." Lorenzo Pellegrini remained the only confirmed absentee. Gasperini described Fenerbahçe as formidable opposition: "They have many important players. They will need time but they have quality." Fenerbahçe manager İsmail Kartal offered warm respect for Roma in return, calling them "the best squad in their history" and noting their three consecutive Serie A victories. Kartal highlighted Roma's man-to-man pressing and ability to move multiple players forward quickly after regaining possession, while confirming Marco Asensio's absence through injury. Defender Milan Skriniar, who had faced Gasperini's Atalanta sides multiple times, emphasised Roma's increased quality under the manager: "With Roma in my opinion the level of quality has risen even more."
Radio Roma analysts debated squad depth before kickoff, with Roberto Pruzzo flagging concerns about midfield options. Several commentators predicted a 1–1 draw, while others backed Roma's chances in Turkey. The coverage also noted that Corbin Friedkin of the ownership group and Vincenzo Montella, now Turkey's national team coach, were in attendance for the fixture, underscoring the significance of Roma's return to European football's elite competition.
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