Roma prepared for their Champions League fixture against Real Madrid at the Stadio Olimpico on 14 October, with thousands of supporters gathering outside the stadium to greet the team. Gates opened at 18:30, with Roma advising fans to arrive 90 minutes before kickoff at 21:00. Head coach Gian Piero Gasperini emphasised the significance of the match, stating Roma must limit Real Madrid's speed, while noting this represented a prestigious opportunity to test themselves against European elite. Gasperini confirmed that Konstantinos Koulierakis had returned to training, though Bryan Cristante and goalkeeper Mile Svilar remained sidelined, with Devis Vásquez replacing Svilar on the UEFA squad list. Tactical preparations suggested potential changes at left-back, with Devyne Rensch potentially replacing Nahuel Molina to provide defensive cover against Real Madrid's attacking threat, though the competition between the two remained open ahead of team sheet confirmation.
Paulo Dybala pledged his commitment to Roma, expressing hope for an Italian ending to his career while praising the club and its supporters for welcoming him. Miralem Pjanic, appearing as a guest analyst, hailed Roma as Serie A's most impressive side under Gasperini and praised Dybala's technical quality as the best in the league. Francesco Totti, attending in person after a two-year absence, backed Roma's title prospects against Inter and credited Gasperini with creating a compact, united squad. José Mourinho, preparing to face his former club, stated before his press conference that Roma and Inter were the strongest in Serie A, and framed the match as one of many in the league phase rather than decisive, expecting both sides to qualify from the competition.
Off the pitch, Roma supporters honoured Mourinho with a banner reading "José, through wounds and victories you taught us a path: to honour Roma to the last madness," displayed at the Colosseum ahead of kickoff. Atlético Madrid fans showed solidarity by unfurling Roma colours during their own Champions League match, continuing a tradition of support between the two sets of ultras. Separately, vandals damaged a mural of Mourinho by spraying paint across the former manager's face. Salvatore Foti, Mourinho's former assistant, recalled their extraordinary bond and the euphoria following Roma's Europa Conference League victory in 2022, contrasting it with the pain of his dismissal eight months later. Fabio Capello offered tactical analysis, identifying Donyell Malen and Mbappé as potential decisive players while flagging Dybala as a danger to Madrid's defence, and noting that Madrid's vulnerabilities at home this season might favour them playing away from the Bernabéu.
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